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WT1 expression in salivary gland pleomorphic adenomas: a reliable marker of the neoplastic myoepithelium.

Langman, Gerald and Andrews, Claire L and Weissferdt, Annikka (2011) WT1 expression in salivary gland pleomorphic adenomas: a reliable marker of the neoplastic myoepithelium. Modern Pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 24 (2). pp. 168-74. ISSN 1530-0285.

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Official URL: http://www.nature.com/modpathol/journal/v24/n2/ful...

Abstract

Pleomorphic adenoma is a benign salivary gland neoplasm with a diverse morphology. This is considered to be a function of the neoplastic myoepithelium, which shows histological and immunophenotypical variability. Wilms' tumor 1 gene (WT1) protein, involved in bidirectional mesenchymal-epithelial transition, has been detected by reverse transcription PCR in salivary gland tumors showing myoepithelial-epithelial differentiation. The aim of this study was to investigate the immunoreactivity of WT1 in pleomorphic adenomas and to compare the pattern of staining with p63 and calponin, two reliable markers of myoepithelial cells. A total of 31 cases of pleomorphic adenoma were selected. The myoepithelium was classified as myoepithelial-like (juxtatubular and spindled), modified myoepithelium (myxoid, chondroid and plasmacytoid) and transformed myoepithelium (solid epithelioid, squamous and basaloid cribriform). Immunohistochemistry for WT1, p63 and calponin was assessed in each myoepithelial component, as well as in nonneoplastic myoepithelial cells and inner tubular epithelial cells. There was no immunostaining of tubular epithelial cells by any of the markers. In contrast to p63 and calponin, WT1 did not react with normal myoepithelial cells. Cytoplasmic WT1 staining was present in all pleomorphic adenomas, and in 29 cases (94%), >50% of neoplastic myoepithelial cells were highlighted. p63 and calponin stained the myoepithelium in 30 tumors. In comparison, 50% of cells were positive in 21 (68%) and 9 (29%) cases of p63 and calponin, respectively. Staining with WT1 showed less variability across the spectrum of myoepithelial differentiation with the difference most marked in the transformed myoepithelium. WT1 is a sensitive marker of the neoplastic myoepithelial cell in pleomorphic adenomas. The role of this protein in influencing the mesenchymal-epithelial state of cells suggests that WT1 and the myoepithelial cell have an important role in the histogenesis of pleomorphic adenomas.

Item Type: Article
Subjects: QY Clinical pathology
Divisions: Clinical Support > Pathology
Depositing User: Mrs Caroline Tranter
Date Deposited: 17 May 2013 08:38
Last Modified: 17 May 2013 08:38
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/86

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