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Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes.

Amos, G C A and Zhang, L and Hawkey, P M and Gaze, W H and Wellington, E M (2014) Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes. Veterinary microbiology, 171 (3-4). pp. 441-7. ISSN 1873-2542. This article is accessible to all HEFT staff and students via NHS Evidence www.evidence.nhs.uk by using their HEFT Athens login Ids

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

The environment harbours a significant diversity of uncultured bacteria and a potential source of novel and extant resistance genes which may recombine with clinically important bacteria disseminated into environmental reservoirs. There is evidence that pollution can select for resistance due to the aggregation of adaptive genes on mobile elements. The aim of this study was to establish the impact of waste water treatment plant (WWTP) effluent disposal to a river by using culture independent methods to study diversity of resistance genes downstream of the WWTP in comparison to upstream. Metagenomic libraries were constructed in Escherichia coli and screened for phenotypic resistance to amikacin, gentamicin, neomycin, ampicillin and ciprofloxacin. Resistance genes were identified by using transposon mutagenesis. A significant increase downstream of the WWTP was observed in the number of phenotypic resistant clones recovered in metagenomic libraries. Common β-lactamases such as blaTEM were recovered as well as a diverse range of acetyltransferases and unusual transporter genes, with evidence for newly emerging resistance mechanisms. The similarities of the predicted proteins to known sequences suggested origins of genes from a very diverse range of bacteria. The study suggests that waste water disposal increases the reservoir of resistance mechanisms in the environment either by addition of resistance genes or by input of agents selective for resistant phenotypes.

Item Type: Article
Additional Information: This article is accessible to all HEFT staff and students via NHS Evidence www.evidence.nhs.uk by using their HEFT Athens login Ids
Subjects: QV Pharmacology
Divisions: Clinical Support > Infection Control
Related URLs:
Depositing User: Mr Philip O'Reilly
Date Deposited: 05 Sep 2014 11:30
Last Modified: 05 Sep 2014 11:30
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/705

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