Confirmation of specificity of reactivity in a solid phase ELISA for the detection of hepatitis E viral antigen improves utility of the assay.

Ankcorn, M J and Ijaz, S and Haywood, B and Neuberger, J and Elsharkawy, A M and Maggs, J and Tedder, R S (2018) Confirmation of specificity of reactivity in a solid phase ELISA for the detection of hepatitis E viral antigen improves utility of the assay. Journal of virological methods, 252. pp. 42-48. ISSN 1879-0984.

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Abstract

Genotype 3 hepatitis E virus (HEV) can lead to persistent infections in immunocompromised hosts. A recently available commercial assay for the detection of HEV antigen (HEV-Ag ELISA, Wantai diagnostics) may enable the study of HEV-Ag dynamics in such persistent infections, however currently there is no confirmatory test available. We generated a putative neutralising reagent from a pool of four convalescent blood donor samples and explored neutralising activity against HEV antigens from clinical samples, HEV tissue-culture and virus-like particles. Using this neutralisation method we were able to differentiate true reactivity from non-specific reactivity in plasma, stool and urine samples. This could also facilitate the introduction of HEV-Ag detection as a screening assay or the study of HEV-Ag in different body fluids.

Item Type: Article
Subjects: QW Microbiology. Immunology
WD Diseases and disorders of systemic, metabolic or environmental origin > WD350 Immunologic diseases
Divisions: Clinical Support > Infectious Diseases
Related URLs:
Depositing User: Jennifer Manders
Date Deposited: 15 Mar 2019 16:08
Last Modified: 15 Mar 2019 16:08
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1926

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