The potential of visible blue light (405 nm) as a novel decontamination strategy for carbapenemase-producing enterobacteriaceae (CPE).

Halstead, Fenella D and Ahmed, Zahra and Bishop, Jonathan R B and Oppenheim, Beryl A (2019) The potential of visible blue light (405 nm) as a novel decontamination strategy for carbapenemase-producing enterobacteriaceae (CPE). Antimicrobial resistance and infection control, 8. p. 14. ISSN 2047-2994. This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs

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Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC63357...

Abstract

Background

Carbapenemase-producing (CPE) pose a considerable threat to modern medicine. New treatment options and methods to limit spread need to be investigated. Blue light (BL) is intrinsically antimicrobial, and we have previously demonstrated significant antimicrobial effects on biofilms of a panel of isolates, including two CPEs.This study was performed to assess the antibacterial activity of 405 nm BL against a panel of CPE isolates (four encoding , three , two , and three encoding both NDM and OXA-48 carbapenemases).

Methods

In vitro experiments were conducted on 72 h old biofilms of CPEs which were exposed to 60 mW/cm of BL. Changes to biofilm seeding were assessed by measuring the optical density of treated and untreated biofilms.

Results

Twelve bacterial clinical isolates (comprising eight , one , and three ) were tested. BL was delivered for 5, 15 and 30 min, achieving doses of 162, 54, and 108 J/cm, respectively.All of the CPEs were susceptible to BL treatment, with increasing reductions in seeding with increasing durations of exposure. At 30 min, reductions in biofilm seeding of ≥80% were observed for 11 of the 12 isolates, compared to five of 12 after 15 min. CPE_8180 was less susceptible than the rest, with a maximum reduction in seeding of 66% at 30 min.

Conclusions

BL is effective at reducing the seeding of mature CPE biofilms in vitro, and offers great promise as a topical decontamination/treatment agent for both clinical and environmental applications.

Item Type: Article
Additional Information: This article is available to all UHB staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their UHB Athens login IDs
Subjects: QW Microbiology. Immunology
Divisions: Clinical Support > Infection Control
Clinical Support > Pathology
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Depositing User: Beth Connors
Date Deposited: 05 Feb 2019 11:07
Last Modified: 05 Feb 2019 11:07
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1823

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