Automated detection of bacterial growth on 96-well plates for high-throughput drug susceptibility testing of Mycobacterium tuberculosis.

Fowler, Philip W and Gibertoni Cruz, Ana Luíza and Hoosdally, Sarah J and Jarrett, Lisa and Borroni, Emanuele and Chiacchiaretta, Matteo and Rathod, Priti and Lehmann, Sarah and Molodtsov, Nikolay and Walker, Timothy M and Robinson, Esther R and Hoffmann, Harald and Peto, Timothy E A and Cirillo, Daniela Maria and Smith, Grace E and Crook, Derrick W (2018) Automated detection of bacterial growth on 96-well plates for high-throughput drug susceptibility testing of Mycobacterium tuberculosis. Microbiology (Reading, England). ISSN 1465-2080.

Full text not available from this repository.
Official URL: http://mic.microbiologyresearch.org/content/journa...

Abstract

M. tuberculosis grows slowly and is challenging to work with experimentally compared with many other bacteria. Although microtitre plates have the potential to enable high-throughput phenotypic testing of M. tuberculosis, they can be difficult to read and interpret. Here we present a software package, the Automated Mycobacterial Growth Detection Algorithm (AMyGDA), that measures how much M. tuberculosis is growing in each well of a 96-well microtitre plate. The plate used here has serial dilutions of 14 anti-tuberculosis drugs, thereby permitting the MICs to be elucidated. The three participating laboratories each inoculated 38 96-well plates with 15 known M. tuberculosis strains (including the standard H37Rv reference strain) and, after 2 weeks' incubation, measured the MICs for all 14 drugs on each plate and took a photograph. By analysing the images, we demonstrate that AMyGDA is reproducible, and that the MICs measured are comparable to those measured by a laboratory scientist. The AMyGDA software will be used by the Comprehensive Resistance Prediction for Tuberculosis: an International Consortium (CRyPTIC) to measure the drug susceptibility profile of a large number (>30000) of samples of M. tuberculosis from patients over the next few years.

Item Type: Article
Subjects: QW Microbiology. Immunology
WC Communicabable diseases
Divisions: Clinical Support
Related URLs:
Depositing User: Miss Emily Johnson
Date Deposited: 31 Oct 2018 16:26
Last Modified: 31 Oct 2018 16:26
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1762

Actions (login required)

View Item View Item