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Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity.

Roberts, Helen M and Grant, Melissa M and Hubber, Naomi and Super, Paul and Singhal, Rishi and Chapple, Iain L C (2017) Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity. Obesity surgery. pp. 1-11. ISSN 1708-0428. This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs

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Official URL: https://link.springer.com/article/10.1007/s11695-0...

Abstract

AIM

The aim of this study was to investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function.

MATERIAL AND METHODS

Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender- and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. PBNs were isolated by density centrifugation and a comprehensive analysis of PBN function was undertaken at various stages of the patients' bariatric surgical care pathway.

RESULTS

Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n = 18, p < 0.001) and release of pro-inflammatory cytokines (n = 10, p < 0.05) and lower PBN extracellular trap (NET) formation (n = 18, p < 0.01). PBN chemotactic accuracy was also impaired prior to surgery (n = 18, p < 0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients.

CONCLUSIONS

Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, alongside inflammation-mediated tissue damage in obese individuals.

Item Type: Article
Additional Information: This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs
Subjects: WO Surgery
Divisions: Planned IP Care > General Surgery
Depositing User: Miss Emily Johnson
Date Deposited: 26 Jan 2018 13:41
Last Modified: 26 Jan 2018 13:41
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1559

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