Comparison of two sample processing methods and nine commercial immunoassays for the detection of interleukin (IL)-1α in the serum of patients with abdominal aortic aneurysm (AAA).

Ahmad, Mehtab and Vohra, Rajiv and Bradbury, Andrew W (2017) Comparison of two sample processing methods and nine commercial immunoassays for the detection of interleukin (IL)-1α in the serum of patients with abdominal aortic aneurysm (AAA). Annals of vascular surgery. ISSN 1615-5947. (In Press) This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs

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Official URL: http://www.annalsofvascularsurgery.com/article/S08...

Abstract

BACKGROUND

For a cytokine to have a role as a clinically useful biomarker it must be measureable in a practical, reliable and reproducible manner. Furthermore, assays from different manufacturers should produce comparable results.

AIM

To examine the effect of two sample processing methodologies and compare nine commercially available immunoassays for their measurement of serum interleukin (IL)-1α in patients with abdominal aortic aneurysm (AAA).

METHODS

Two sample processing methodologies and nine manufacturers' immunoassays were compared. Each immunoassay was also tested for detection of both IL-1α isoforms.

RESULTS

A positive signal for IL-1α was found in all serum samples, in all immunoassays, using both processing methods. In the majority, titre concentrations were unquantifiable with values below manufacturers' detectable range. Variability in titre concentrations was seen across all immunoassays. With the exception of one immunoassay, all were able to detect both IL-1α isoforms.

CONCLUSIONS

Researchers wishing to measure serum cytokines levels should be aware that differences in sample processing methods and manufacturers' immunoassays can affect results. This may result in misleading conclusions being drawn about biological processes underpinning a wide range of inflammatory diseases.

Item Type: Article
Additional Information: This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs
Subjects: WG Cardiovascular system. Cardiology
Divisions: Planned IP Care > General Surgery
Planned IP Care > Vascular
Related URLs:
Depositing User: Mrs Semanti Chakraborty
Date Deposited: 17 Apr 2018 15:46
Last Modified: 17 Apr 2018 15:46
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1557

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