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Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays.

Ajileye, Adebisi and Alvarez, Nataly and Merker, Matthias and Walker, Timothy M and Akter, Suriya and Brown, Kerstin and Moradigaravand, Danesh and Schön, Thomas and Andres, Sönke and Schleusener, Viola and Omar, Shaheed V and Coll, Francesc and Huang, Hairong and Diel, Roland and Ismail, Nazir and Parkhill, Julian and de Jong, Bouke C and Peto, Tim E A and Crook, Derrick W and Niemann, Stefan and Robledo, Jaime and Smith, E Grace and Peacock, Sharon J and Köser, Claudio U (2017) Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays. Antimicrobial agents and chemotherapy, 61 (4). ISSN 1098-6596. This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs

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Official URL: http://aac.asm.org/content/61/4/e02169-16.long

Abstract

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Item Type: Article
Additional Information: This article is available to all HEFT staff and students via ASK Discovery tool http://tinyurl.com/z795c8c by using their HEFT Athens login IDs
Subjects: QW Microbiology. Immunology
WF Respiratory system. Respiratory medicine
Divisions: Clinical Support > Pathology
Related URLs:
Depositing User: Mrs Yolande Brookes
Date Deposited: 23 Nov 2017 12:38
Last Modified: 23 Nov 2017 12:38
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1491

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