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Verification of the Cepheid Xpert Carba-R assay for the detection of carbapenemase genes in bacterial isolates cultured on alternative solid culture media.

Tanner, Hannah E (2017) Verification of the Cepheid Xpert Carba-R assay for the detection of carbapenemase genes in bacterial isolates cultured on alternative solid culture media. The Journal of hospital infection. ISSN 1532-2939. This article is available to all HEFT staff and students via ASK HEFT Discovery tool http://tinyurl.com/z795c8c using their HEFT Athens Login.

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Official URL: http://www.journalofhospitalinfection.com/article/...

Abstract

The Xpert Carba-R (Cepheid) is a PCR assay for detection and differentiation of five common carbapenemase genes. If used according to manufacturer's instructions, bacterial isolates tested must be cultured on blood or MacConkey agar. This study confirms that the assay performs well against a diverse panel of bacterial isolates with known carbapenemase genes. It also demonstrates that the assay performs well using three solid agar culture media that have not been recommended by the PCR assay's manufacturer: CLED, ChromID CARBA-SMART, and a nutrient slope. By testing isolates directly from any of these media, delays in laboratory reporting can be avoided.

Item Type: Article
Additional Information: This article is available to all HEFT staff and students via ASK HEFT Discovery tool http://tinyurl.com/z795c8c using their HEFT Athens Login.
Subjects: QY Clinical pathology
Divisions: Clinical Support > Pathology
Related URLs:
Depositing User: Mr Philip O'Reilly
Date Deposited: 15 Nov 2017 15:48
Last Modified: 15 Nov 2017 15:48
URI: http://www.repository.heartofengland.nhs.uk/id/eprint/1439

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